RJAVS Jan-June 2018
 
 
 

 


Study of genetic polymorphism in BMPRIB gene and its association with improving twin production in Egyptian sheep and goats

Ibrahim M. Farag, Hassan R. Darwish, Dalia M. Aboelhassan, Mohamed M. Aboelenin, Shakweer W.M.E

Abstract: The present research was carried out to investigate the genetic polymorphism in BMPRIB (FecB) gene and to test whether this polymorphism is associated with improving twin production in Egyptian domestic sheep and goats. Blood samples were collected from 139 animals including 95 of sheep and 44 of goats. These animals involved 113 mothers (83 of sheep and 30 of goats) at the first, second, third and fourth parity. The other remaining 26 females (12 of sheep and 14 of goats) were at sexual maturity age to detect and select the females that carry desirable gene markers for their using in successful breeding program. Genomic DNA was extracted from whole blood samples. PCR was carried out for amplification of a fragment with 140 bp of FecB locus. PCR-RFLP, PCR-SSCP and nucleotide sequence analysis were performed to detect and identify the genetic polymorphism of the candidate gene. Results of PCR-RFLP analysis observed no genetic polymorphism in FecB locus. However, PCR-SSCP analysis clarified genetic polymorphism with two patterns (P1 and P2) in sheep. Pattern 1 was detected in 12 animals including 11 mothers and one female at age of sexual maturity. Pattern 2 was observed in 83 animals involving 72 mothers and 11 females at age of sexual maturity. All goat animals were monomorphic and they were identical with P2 of sheep. The mothers with P1 had more percentage of twin production and increase the mean number of lambing than mothers with P2 and statistical analysis showed that there were significant differences between the two patterns. Sequence analysis determined two types of alleles, mutant allele A and wild type allele T with two genotypes AT and TT. Genotype AT was noted to be associated with improving twin production and increase the mean number of lambing in comparison with TT genotype. In conclusion, the present research clarified that polymorphism in FecB locus of BMPRIB gene is a favorable genetic marker that influences the fecundity of Egyptian small ruminant especially in sheep.


[ FULL TEXT PDF 1-5 ] DOI: 10.22587/rjavs.2018.10.1.1


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Effect of genetic polymorphism of BMP15 gene on improving twin production in Egyptian small ruminants

Ibrahim M. Farag, Dalia M. Aboelhassan, Inas S. Ghaly, Hassan R. Darwish, Ahmed M. Darwish and Mariam G. Eshak.

Abstract: The present work was designed to study the genetic polymorphism in BMP15 gene at four sites, FecXB, FecXG, FecXH and FecXI and its association with improving twin production of Egyptian sheep and goats. This study was performed on 139 females including 113 mothers (83 of sheep and 30 of goats) at first , second, third and fourth parity and 26 animals (12 of sheep and 14 of goats) at age of sexual maturity to quantify the females that have favorable gene marker for their utilizing in successful breeding program. Sheep breeds involved Barki, Osseimi, Rahmani, Saudanez and Awase, whereas, goats breeds involved Zaraibi, Damascus, Boer, Sanan and Barki. Blood samples were collected from above all animals and genomic DNA was extracted. The fragments at size of 153 bp, 141 bp, 240 bp and 154 were amplified in PCR conditions for FecXB, FecXG, FecXH and FecXI, respectively. PCR-RFLP, PCR-SSCP and nucleotide sequence analyses were investigated. PCR-RFLP analysis showed no genetic polymorphisms in such four sites of BMP15 gene. However, PCR-SSCP analysis revealed genetic polymorphisms at two patterns for three sites, FecXB, FecXG and FecXH in sheep animals, whereas, all goat animals were monomorphic. Also, PCR-SSCP detected genetic polymorphism at two patterns (P1 and P2) for one site FecXI in goat animals, however, all sheep females in such site were monomorphic. Pattern P2 of each FecXB and FecXH loci in sheep had high rates of twin production and increase the mean number of lambing as compared to pattern P1, whereas, in the two patterns of FecXG, one of them was only observed in one mother of sheep with three single births. Concerning the two patterns in FecXI of goats, the mothers in one pattern were observed to give high rates of twin production and increase the mean number of lambing with respect to other pattern. Nucleotide sequence analysis quantified two types of alleles (mutant and wild alleles with two genotypes in each mentioned four sites of BMP15 gene. Mutant and wild type alleles were G-A, T-A, A-C and T-C with genotypes AG and AA, TA and AA, AC and CC and CT and CC in FecXB, FecXG, FecXH and FecXI sites, respectively. AG (in FecXB site) and AC (in FecXH site) genotypes of sheep and CT (in FecXI site) genotype of goats were favorable genetic markers, where these genotypes were discriminated with improving twin production and increase the mean number of lambing (or kidding). However, TA genotype of FecXG was found in one mother of sheep, this mother was revealed to give three single births. In conclusion: the present work proved that BMP15 gene is a major gene that affects prolificacy in Egyptian small ruminants. Therefore, the genetic polymorphism in such gene is considered to be a desirable genetic marker that could be revealed prior using in successful breeding program. This program can utilize in development of new prolific breeds of small ruminant species.

[ FULL TEXT PDF 6-12 ]
DOI: 10.22587/rjavs.2018.10.1.2

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Blood biochemical changes and thyroid hormones pattern of Barki ewes as affected by biological supplementation under semi-arid conditions of Egypt

M. F. El-Bassiony, A. S. El-Hawy, Reda, A. Abd-Elazem, A. Abdou

Abstract This study was carried out to investigate the effect of different levels of biological additives (ZAD® and ZADO®) on some blood biochemical parameters, as well as, thyroid hormones pattern of Barki ewes during different physiological stages. Seventy five synchronized pregnant Barki ewes were randomly allotted into five equal groups (15 each). The 1st group (G1) served as control and fed on concentrate feed mixture (CFM), the 2nd and 3rd groups (G2 and G3) were fed CFM with 6 and 10 g/h/d from ZADO®, respectively. While, the 4th and 5th groups (G4 and G5) were fed CFM with 6 and 10 ml/h/d ZAD®, respectively. All groups were offered bean (Phaseolus vulgaris) straw ad libitum. Blood biochemical parameters (total proteins, liver enzymes activities, kidney functions, glucose and cholesterol) concentrations were determined. Triiodothronine (T3), thyroxin (T4) levels were also measured during the experimental period. Results indicated that total protein, albumin, globulin and glucose concentrations were significantly higher in all treated groups compared to the control group. Albumin/globulin (A/G) ratio was significantly lower in G3 compared to the other groups. Cholesterol concentration was higher (P˂0.05) in ZAD groups (G4 and G5) than other groups. Blood urea nitrogen showed slightly increase in the control group than treated groups, while creatinine concentration increased insignificantly in G4. Liver enzymes activities (AST and ALT) showed a significant increase in ZADO and ZAD groups compared to the control one, Thyroid hormones (T3 and T4) tended to increase in all treated groups compared to the control. Early lactating ewes recorded the highest concentrations (P˂0.05) of TP, Glo, T3 and T4. Conversely, late pregnant ewes recorded the highest values of Alb, Cho, Cr and ALT. It could be concluded that ZAD® and ZADO® have positive effects on some blood biochemical and hormonal pattern which positively reflected on physiological responses.

[ FULL TEXT PDF 15-24 ] DOI: 10.22587/rjavs.2018.10.1.3

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Biochemical, hematological, immunological responses and growth performance of Barki lambs born to ewes fed on Nigella sativa meal

A. S. El-Hawy

Abstract The objective of this research was to investigate the effect of feeding Nigella sativa meal as an alternative source of protein of Barki ewes on the productive performance, some blood biochemicals, immunological and hematological responses of Barki ewes and their lambs. Sixty adult Barki ewes (2.0-3.0 years old and 38.89 ± 1.02 kg average body weight) were randomly assigned into equal three groups (20 each). The first group (G1) served as control and fed the basal diet contain 20% cotton seed meal and 6% soya bean meal as a source of protein, while the second group (G2) fed diet contained 13.5% of Nigella sativa meal (NS meal) and 8% soya bean meal. The Third group (G3) fed diet contained 25% of NS meal as the lonely source of protein. Blood samples were taken from each ewe at the beginning of the experiment and before lambing. For lambs, five males of each group were randomly assigned for blood sampling at 30, 60 and 90 days after birth. Some blood biochemicals, immunological, hematological and thyroid hormones (T3 and T4) were determined. Number of lambs born and weaned alive, mortality rate from birth to weaning, birth and weaning weights and average daily gain were also recorded. Birth and weaning weights were higher (P<0.05) in lambs born to ewes fed on Nigella sativa meal. Values of birth weights were 3.01, 3.31 and 3.44 for lambs of G1, G2 and G3, respectively. The corresponding values of weaning weights were 20.56, 21.88 and 23.00 kg, respectively. Moreover, average daily gain was significantly increased in lambs of G3 than those in G1 and G2 groups. At weaning, number of kilograms weaned per ewe lambed were 18.27, 20.66 and 20.29 kg in G1, G2 and G3, respectively. Milk yield was insignificantly increased in NS meal groups than the control one. In ewes, liver enzymes, T3 and T4 were not affected by treatment. Ewes fed NS meal had higher (P<0.05) values of white blood cells, package cell volume, plasma total protein, globulin, Immunoglobulin A (IgA) and total antioxidants (TAO). For offsprings, all haematological (WBCs, RBCs, Hb and PCV) and immunological parameters (IgA, IgG, IgM and TAO) were increased in lambs of G3 and G2 as compared to control ones. Also, T4 concentration showed higher values more than lambs of control group. However, plasma proteins, liver enzymes and T3 were not affected by treatment. From above results, it could be concluded that using Nigella sativa meal as a source of protein in rations of ewes, improved birth and weaning weights of their lambs, in addition to increasing their immunological status.

[ FULL TEXT PDF 25-36 ] DOI: 10.22587/rjavs.2018.10.1.4

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Productive and reproductive performance of Barki ewes fed on sprouted barley grains on desert by-products during lactating period

Helal, H.G.

Abstract In arid and semi-arid areas browse species such as Acacia saligna is a leguminous shrub was considered one of the principal feed resources, barley straw one of agriculture wastes produced in a large amount but they have low nutritive value, so several treatments were applied to ameliorate the utilization of acacia and barley straw. The present experiment was conducted to investigate the effect of sprouted barley grains on acacia pruning and barley straw as media and mixture of them. Forty-four Barki ewes were randomly divided into four equal groups (11 ewes each). Ewes of the first group were fed a control diet of berseem hay and CFM, the second group (D1) was fed ad libitum a diet contained sprouted barley grains on barley straw with CFM, the third group (D2) was fed ad libitum a diet contained sprouted barley grains on acacia pruning with CFM, and the fourth group was fed ad libitum a diet contained sprouted barley grains on mixture of similar amounts of both barley straw and acacia pruning with CFM. Ewes fed the three experimental fodders recorded higher (P<0.05) dry fodder intake (g/h/d) than those fed control diet. Digestibility of CP for lactating Barki ewes was not affected by the type of roughage while the values of DM, OM, CF, NDF and ADF digestibility were (P<0.05) higher with sprouted barley seeds (D2, D1 and D3) than those fed the control diet. Also, total digestible nutrients (TDN%) showed higher (P<0.05) improvements for ewes fed D2 followed by control diet, D3 and the lowest was D1. However, There were insignificant differences between treatment groups in digestible crude protein (DCP%) values. Nitrogen balance showed (P<0.05) an increased in D2 diets compared to other diets. No significant differences were noted among tested groups for body weight changes, pH and ammonia-nitrogen (NH3-N), but lactating ewes fed sprouted barley grains on roughages showed higher (P<0.01) total volatile fatty acid (TVFA´s) than ewes fed the control diet. Milk composition of fat, total solids (TS), ash and energy (kcal/kg) had higher (P<0.01) values for ewes fed D2 diet compared to other groups, while milk yield and 4% fat-corrected milk (FCM) were not affected. The findings of the present study showed (P<0.01) increased on average birth weight for lambs fed D2 diet compared to other groups. Average daily gain was insignificantly higher for animals fed D2 diet than those fed the control diet. Blood parameters were not affected by treatments. The lowest total feed cost along the feeding period was observed for animals fed sprouted fodder compared to the control diet. In conclusion we can produce green fodder by utilizing dried acacia pruning and barley straw by simple methodology using crop sprouts (barley) to improve productive performance for of Barki ewes.

[ FULL TEXT PDF DOI: 37-48 ] DOI: 10.22587/rjavs.2018.10.1.5

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