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Prevalence of Multidrug-Resistant Klebsiella Pneumoniae Infection in the Intensive Care Unit

Khaled Khuzim, Atif Bamagoos, Mohamed Morsi M. Ahmed

ABSTRACT: In hospitals, K. pneumoniae is widely regarded as the most common opportunistic pathogen. The goal of this study was to confirm the hypothesis that K. pneumoniae is a major cause of HAIs and has high antibiotic resistance. A variety of clinical samples were collected and processed for K. pneumoniae isolation using selective culture media from 264 patients at Asis Central who were suspected of having K. pneumoniae. The positive isolates were then tested using the Phoenix 100TM automated system for the presence of drug resistance and virulence genes. Bacteria were isolated from 158 (50.8%) of the 264 collected samples. The vast majority of bacteria discovered (83%) came from a single isolate, with the remainder (17%) coming from a variety of sources. Female samples had a statistically significant increase in bacterial isolates when compared to male samples (P=0.026). More than 78 percent of the bacteria tested positive for antibiotic resistance in the study. The resistance rates for gentamicin, levofloxacin, amikacin, minocycline, and norfloxacin were 100%, 96.9%, 94.5 percent, 87.1 percent, and 83.6 percent, respectively. The Xpert Carba-R assay revealed that 23 (8.7%) of the samples tested positive for target genes; these samples were blaNDM1 (n=7) and bla OXA48 (n=16) resistant. The study's findings could be used to create a hospital antibiotic policy to prevent the spread of multidrug-resistant strains.

[ FULL TEXT PDF 1-9 ] DOI: 10.22587/jasr.2023.19.2.1

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