AEB Volume 14, Number 3: March, 2020

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Differential RNA editing of mitochondrial atp1 gene in Catharanthus roseus tissues

Huda Alhamdan, Wasimah B Alshammari, Thana Khan, Sherif Edris, Sameh E. Hassanein, Ahmed M Ramadan

Abstract RNA editing is a posttranscriptional modification. This modification is executed by insertion, deletion or nucleotide substitution. ATP synthase subunit1 (ATP1) gene is encoded to the alpha subunit of complex V which is indispensable for generating mitochondrial ATP. RNA editing in this gene was investigated in many plants like Arabidopsis and Gossypium hirsutum. In this study, five RNA editing sites were in ATP1 gene transcripts in different Catharanthus roseus tissues using high throughput sequencing. Editing sites (C1039, C1178, C1292, C1415, and C1490) were identified from C to T substitution in C. roseus tissues. Results indicate that RNA editing in ATP1 varies across C. roseus tissues. Three edits of ATP1 gene transcripts (C1039, C1292 and C1415) were detected in all C. roseus tissues. The most frequent number of edits appeared in flower and leaf ATP1 tissues of C. roseus compared to other tissues. A C1178 edit of ATP1 transcript was only observed in flower, leaf and hairy root of C. roseus, whereas it was absent in root, stem and seedling. Furthermore, C1490 site of this gene was recognized in all C. roseus tissues except in hairy root tissue. Differential editing of ATP1 gene transcript might control the rate of ATP production.

[ FULL TEXT PDF 1-9 ] DOI: 10.22587/aeb.2020.14.3.1


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Utilization of White Teak Sawdust Waste (Gmelina Arborea Roxb.) as Biodegradable Plastic

Achmad Amiruddin, Muhammad Farid Samawi, Maming

Abstract The accumulation of plastic waste made from synthetic polymers for environmental pollution is needed on the defenders of life that are around it, so that to overcome them, it is needed plastic that is easily biodegradable and does not need environmental pollution. Polymers that can be used are natural polymers needed from the agricultural sector such as teak. This study aims to synthesize biodegradable plastics released from natural polymers containing cellulose fibers contained in waste teak sawdust (Gmelina arborea Roxb.) and compare cellulose masses based on optimal white teak sawdust in making biodegradable plastics. The stages of this research are cellulose extraction, making biodegradable plastics and testing the characteristics of biodegradable plastics (mechanical tests and FTIR analysis). The results showed that cellulose mass based on optimal white teak sawdust in making biodegradable plastics was 0.8 grams using chitosan and sorbitol plasticizers. The optimal tensile strength test result is 5.0449 N / mm2 with 14.81% elongation percent.

[ FULL TEXT PDF 10-15 ] DOI: 10.22587/aeb.2020.14.3.2

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Effect of Heavy Metals Accumulation of Cadmium (Cd) on Leaf Chlorophyll Green Champa (Polyaltia longifolia) in Industrial Area Makassar, South Sulawesi

Hardiyanti YM, Fahruddin Fahruddin, Paulina Taba

Abstract Heavy metal pollution in the industrial area to cause problems on the vegetation growing around it. The study aims to determine the content of heavy metals Cd accumulates in the leaves Polyaltia longifolia in the industrial area Makassar. Concentrations of heavy metals Cd measurement with AAS method and the measurement of chlorophyll concentration was measured using a spectrophotometer at wavelength 649 and 665 nm, and the results showed the concentration of heavy metals Cd accumulates in leaves Polyaltia longifolia is Cd is from 0.035 to 0.077 g / g at (leaves 5-10 cm of the lower position and leaves the upper position as high as 10-25 m. Then the chlorophyll concentration is 13.51 - 26.83 ug /g (chloropyll leaves 5-10 cm of the lower position and leaves the upper position as high as 10-25 m. heavy metals Cd affect the levels of chlorophyll in the leaves Polyaltia longifolia (leaf upper position) that F.hitung > of the F.tabel with a value of 0.310 > 0.05 means did not show any significant damage (> 0.05). While, heavy metals Cd in Polyaltia longifolia leaf (leaf lower position) that F.hitung <of the F.tabel with a value of 0.02 < 0.05 means shows the damage significantly.

[ FULL TEXT PDF 16-20 ] DOI: 10.22587/aeb.2020.14.3.3

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