AEB Volume 15, Number 12: December, 2021


Tissue culture technique on micropropagation of grape B41

Mayada Ahmad Al-Sameen, Khalil Al-Ma’ari, Fahd Al-Bisky

Abstract: Research has been carried out to evaluate the growth dynamics of lactic acid bacteria (LAB) Lactobacillus plantarum (L.plantarum) using collagen extract (CE) from the broiler claw skin (BCS) as a substrate. The bacterial dynamics studied were related to total lactic acid (TLA) production, pH, dissolved protein (DP) and bacterial growth (BG) during the fermentation process. A total of 50 g of BCS was used with fermentation time (24; 48 and 72 hours). The results was showed that fermentation time using L.plantarum as a culture with CE from BCS as a substrate did not affect on bacterial dynamics (TLA, pH, dissolved protein and BG) during the fermentation process. Using L.plantarum with EC from BCS as substrate can be applied with 24 hours fermentation time. The bacterial dynamics produced by the fermentation time were 2.35±0.50%; 5.88±0.10; 45.60±2.21 mg/mL and 15.591±0.92 Log10 CFU/mL respectively.

[ FULL TEXT PDF 1-6 ] DOI: 10.22587/aeb.2021.15.12.1


Screening Different Desert Soils in Makkah Province to Detect Novel Antibiotics Producing Actinomycetes

Abdullah A I Hausawi, Nabih A Baeshen, Ahmed Mahmoud Al-Hejin; Mohammed A. Al-Matary; Saleh M. Almaaqar and Mohamed Morsi Mohamed Ahmed

Abstract The purpose of this study was to isolate, purify, and characterize actinomycetes from desert soil surrounding some plants scattered in Makkah Region using biochemical, morphological, and 16S rRNA gene sequencing techniques, as well as to conduct a taxonomic study of species that show activity against some pathogens and to study the characteristics and properties of antibiotics extracted. Soil samples were collected from three locations in Saudi Arabia's Makkah region. They were brought to the lab and cultured for four days at 27° C using actinomistate isolation media. We obtained eight samples based on physiological, morphological, and 16S rRNA gene sequences: Streptomyces spiralis, Streptomyces spinoverrucosus, Streptomyces fimbriatus, Streptomyces carpinensis, Streptomyces geysiriensis, Streptomyces djakartensis, Streptomyces levis, and Streptomyces atacamensis. All isolates of Streptomyces demonstrated antibacterial efficacy against Streptococcus pneumonia, Methicillin-resistant Staphylococcus aureus, Staphylococcus aureus, Streptococcus pyogenes, and antifungal including Aspergillus niger and Candida albicans. These isolates had antimicrobial efficacy and might be used to create novel antibiotics for medicinal or agricultural application.

[ FULL TEXT PDF 7-13 ] DOI: 10.22587/aeb.2021.15.12.2


Microbial & Molecular Identification of Microbiota of Processed Chicken Products

Ahmed Talat Mohamed Ali Nemer, Mohamed H. Z. Mutwakil, and Mohammed Al-Matary

Abstract: Poultry meat and meat products are the major sources of animal protein in the human diet throughout the world; however, they can be contaminated by various microorganisms. In this study, we isolated bacterial strains from chicken nuggets, chicken burgers, chicken franks, chicken balls, chicken kebab, chicken mince (frozen), and chicken mortadella purchased from two different retail stores located in Makkah. Bacterial strains were then characterized based on their biochemical features and 16S rRNA gene phylogeny. The isolates were also examined for their susceptibility to antibiotics. In this study, a total of fifty-one strains were isolated. Among them, forty-two strains were gram-positive and only nine strains were gram-negative bacteria. Thirty-two strains were oxidase-positive, and forty-two isolates were catalase-positive. 16S rRNA gene phylogeny showed that the bacteria belonged to sixteen genera, including Neisseria, Brevibacillus, Bacillus, Exiguobacterium, Rothia, Staphylococcus, Lysinibacillus, Kocuria, Sphingomonas, Novosphingobium, Enterococcus, Pelomonas, Paenibacillus, Klebsiella, Morganella, and Lactococcus. Bacterial strains responded differently against different antibiotics. The isolates A12 and A34 showed resistance to the highest number of antibiotics tested. Overall, the present study revealed that various poultry meat products in the studied area were contaminated by bacterial populations, and therefore, it is essential to investigate stores regularly to reduce microbial contamination in order to ensure public health.

[ FULL TEXT PDF 14-22 ] DOI: 10.22587/aeb.2021.15.12.3


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