AEB Volume 13, Number 4 : April, 2019
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The Antibacterial Activity of a Fraction Extracted from Camel Urine against Mycobacterium tuberculosis Isolated from Tuberculosis Patients in Jeddah City

Raniya Sami Bakhsh, Samah Omar Noor, Faten Abdul Rahman Khorshid, Faten Ahmed Alsulaimany, Abdulwahab Alzahrani, Azhar Abdullah Najjar, Ahmed M.M. Al-Hejin

Abstract This study aimed to investigate the effect of PMF (Prophet Medicine fraction) an extraction from camel urine against Mycobacterium tuberculosis bacteria (MTB) that causes Tuberculosis (TB) disease and determine the period of treatment required for effectiveness comparing with Rifampicin (RIF) antibiotic. This study depended on an experimental design that used 10 random samples of MTB from King Fahad Armed Hospital (KFAH) Processed. The study was conducted in two auto-analyzer systems BACTEC 960 culture system and GeneXpert MTB/RIF assay with five different PMF concentrations. In total, all concentrations of PMF drug except 10% compared with the RIF antibiotic, showed some effectiveness on MTB. In summary, these results showed a non-significant effect of the PMF concentrations except 10% that indicated as the ideal concentration of this drug that reduces the number of bacteria. Further statistical tests revealed the P-value of the F test was less than 0.05 %. Overall, these results indicate no complete effect comparison between growth control of MTB treated with RIF and treated with PMF except 10% that need further studies.

[ FULL TEXT PDF 1-6 ] DOI: 10.22587/aeb.2019.13.4.1

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Molecular Identification for Camel Udder Microbiota

Zayed Alnefaie, Mohamed H. Mutawakil and Mohamed Morsi M. Ahmed

Abstract Dromedary camel (Camelus dromedarius) can survive and deliver the deliberate significant measure of drain amid intermittent and delayed hot and dry condition. Camel drain is considered a standout amongst the essential nourishment sources because of its wholesome esteem. Intermammary contamination and low measures are the principal factors that undermined the camel mammary organ. The purpose of our study was to isolate pathogenic bacteria present in camel's udder. In this study both biochemical and modern technique 16s rRNA gene analysis was used. 100 swaps samples were taken from five camels breeds. Samples were analyzed aerobically and anaerobically to isolate the bacterial strains. In all 100 swaps, we found 35 pathogen bacteria. So, our study revealed that molecular techniques such as 16s rRNA are a powerful tool to detect pathogenic bacteria in camel’s udder.

[ FULL TEXT PDF 7-12 ] DOI: 10.22587/aeb.2019.13.4.2

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Antioxidant Activity and Rancidity of Kefir Face Mask “Bedak Lotong” Incorporated with Various Levels of Temulawak (Curcuma xanthoriza Roxb)

Dewi Ramadani, Ratmawati Malaka and Fatma Maruddin

Abstract Formation of free radicals can be reduced with application of antioxidants, such as vitamin E, carotene, vitamin C, as well as usage of natural materials and chemicals capable of scavenging the radicals. Phenolic compounds are well known to exert antioxidative properties. “Bedak Lotong”, a traditional product,was rich in phenols, derived from its natural ingredients such as black glutinous rice (Oryza Sativa L.glutinosa), temulawak (Curcuma xanthoriza Roxb), and tamarind (Tamarindus indicia L.). This present work aimed to evaluate antioxidant activity and TBA value of kefir mask “Bedak Lotong”. One way-completely randomized design was arranged, with percentage of temulawak (0%, 15%, 30%, and 45%) as a single factor. The results demonstrated that higher proportion of temulawak added in kefir mask “Bedak Lotong” could produce a higher antioxidant activity, which in turn, leads to reduction of TBA value, indicating that fat oxidation or rancidity was reduced.

[ FULL TEXT PDF 13-17 ] DOI: 10.22587/aeb.2019.13.4.3

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The Role of Propioni Bacterium acnes in Acne Vulgaris

Neama Ahmad Al-Nabati, Ahmed Mahmoud Al-Hejin, Nouf Talal Mleeh, Noor Mohammed Bataweel, Mohamed Abu-Zaid, Samah Omar Noor, Maged Mostafa Mahmoud, Azhar Abdullah Najjar, Mohamed M. M. Ahmed

Abstract Acne vulgaris is an inflammatory disease caused by opportunistic microorganism namely Propionibacterium acnes which thrive on the sebum and uses the nutrition’s to develop the condition. P. acnes is Gram-positive, non-spore-forming, non-motile, rod-shaped bacterium, microaerophilic and anaerobic bacilli that grow best in anaerobic conditions under limited oxygen concentrations. This paper discusses the role and characteristics of P. acnes in the pathogenesis of acne disease. The mechanism involved in acnes include; abnormal production of sebum, abnormal keratinization, an inflammatory immune response, hormonal changes and a variety of mutation in genes such as polymorphisms in IL-1α, TNF-α, and CYP1A1. Although the bacteria are part of normal microbiota, some studies linked P. acnes to other diseases some are corneal ulcers, endocarditis, sarcoidosis, cholesterol gallstones, hyperostosis, inflammation of lymph nodes, lungs, eyes, liver and osteitis (SAPHO) syndrome. The incidence of prevalence of antibiotic-resistant strains up to date is increasing, some of reasons is the characteristics of the bacteria such as biofilm formation, and phagocytosis. Studies to date investigated the role of P. acnes in acne vulgaris, until now, the relationship between acne and the bacterium is still in debate.

[ FULL TEXT PDF 18-23 ] DOI: 10.22587/aeb.2019.13.4.4

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